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An evaluation of in vivo voltage-sensitive dyes: pharmacological side effects and signal-to-noise ratios after effective removal of brain-pulsation artifacts.

Author: DevonshireI M, GrandyT H, GreenfieldS A

Journal ArticleAn article published in an academic or professional journal that reports original research or scholarly analysis.
Research Support, Non-U.S. Gov'tResearch funding provided by government agencies outside the United States.

Overview

This study investigated the pharmacological side effects and signal-to-noise ratios (SNRs) of two commonly used voltage-sensitive dyes (VSDs): RH-1691 and di-4-ANEPPS. The study found that RH-1691 caused a significant and prolonged increase in SEP amplitude, while di-4-ANEPPS did not. The study also found that di-4-ANEPPS produced no detectable neurophysiological changes in the system under investigation.
Paper Details　
Original Abstract of the Article :
In the current study, we investigated pharmacological side effects and signal-to-noise ratios (SNRs) of two commonly used voltage-sensitive dyes (VSDs): the blue dye RH-1691 (1 mg/ml) and the red dye di-4-ANEPPS (0.1 mg/ml), applied in vivo to the rat barrel cortex. Blue dyes are often favored over ...See full text at original site
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引用元：
https://doi.org/10.1152/jn.00512.2011

データ提供：米国国立医学図書館（NLM）

In Vivo Voltage-Sensitive Dyes: A Deeper Look at Their Use

Voltage-sensitive dyes (VSDs) are valuable tools for studying neuronal activity in real time. This study investigated the pharmacological side effects and signal-to-noise ratios (SNRs) of two commonly used VSDs, RH-1691 and di-4-ANEPPS, in the rat barrel cortex. The researchers found that RH-1691 significantly increased somatosensory-evoked potential (SEP) amplitude, suggesting potential pharmacological side effects. In contrast, di-4-ANEPPS did not influence SEP amplitude or spontaneous cortical activity, making it a potentially safer and more reliable option for in vivo VSDI recordings. This study provides valuable insights into the specific characteristics and potential limitations of different VSDs.

Di-4-ANEPPS: A More Reliable Choice for In Vivo VSDI

The study's findings suggest that di-4-ANEPPS is a more suitable choice for in vivo VSDI recordings compared to RH-1691. Its lack of significant pharmacological side effects and improved SNR after artifact removal make it a more reliable tool for studying neuronal activity. This study provides researchers with valuable information for choosing the appropriate VSD for their research and ensuring the accuracy and reliability of their data.

Navigating the Landscape of Neuronal Research: Choosing the Right Tools

The field of neuronal research is a vast and ever-evolving landscape, like a desert with shifting sands and hidden treasures. This study, like a skilled explorer, guides us through the nuances of using VSDs for studying neuronal activity. It underscores the importance of carefully evaluating the specific characteristics and potential limitations of different tools to ensure accurate and reliable data collection.

Dr. Camel's Conclusion

The journey of neuronal research is a long and winding path, filled with both challenges and rewards. This study, like a well-worn caravan route, provides valuable insights into the world of VSDs, helping us to choose the right tools and navigate the complexities of this fascinating field. It reminds us that a careful and thoughtful approach is essential for uncovering the mysteries of the brain and its intricate workings.
Date :
  1. Date Completed 2013-05-20
  2. Date Revised 2012-12-03
Further Info :

Pubmed ID

22972958

DOI: Digital Object Identifier

10.1152/jn.00512.2011

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