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1,6-Hexanediol, commonly used to dissolve liquid-liquid phase separated condensates, directly impairs kinase and phosphatase activities.

Author: DüsterRobert, GeyerMatthias, KaltheunerInes H, SchmitzMaximilian

Research Support, Non-U.S. Gov'tResearch funding provided by government agencies outside the United States.
Journal ArticleAn article published in an academic or professional journal that reports original research or scholarly analysis.

Overview

1,6-Hexanediol is widely used to dissolve LLPS assemblies in phase separation studies. However, this study found that 1,6-hexanediol can significantly impair kinase and phosphatase activity at concentrations commonly used to dissolve LLPS droplets (5-10%). This suggests that 1,6-hexanediol may have unintended effects on cell function and should be used cautiously in phase separation studies.
Paper Details　
Original Abstract of the Article :
The concept of liquid-liquid phase separation (LLPS) has emerged as an intriguing mechanism for the organization of membraneless compartments in cells. The alcohol 1,6-hexanediol is widely used as a control to dissolve LLPS assemblies in phase separation studies in diverse fields. However, little is...See full text at original site
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引用元：
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7948595/

データ提供：米国国立医学図書館（NLM）

1,6-Hexanediol: A Common Solvent With Potential Side Effects

The concept of liquid-liquid phase separation (LLPS) has emerged as a fascinating mechanism for organizing membraneless compartments within cells. 1,6-hexanediol is frequently used as a control agent to dissolve LLPS assemblies in phase separation studies. However, this research explores the potential side effects of 1,6-hexanediol, highlighting its impact on the activity of various enzymes. The authors raise concerns about the potential for 1,6-hexanediol to compromise data interpretation and mislead scientific understanding in phase separation research.

Navigating the Complexities of Phase Separation Research

This study reminds us of the importance of carefully considering the potential side effects of commonly used reagents in scientific research. While 1,6-hexanediol has proven useful in dissolving LLPS assemblies, this research demonstrates its potential to interfere with enzymatic activity, particularly kinases and phosphatases. The authors urge researchers to exercise caution and explore alternative methods to minimize potential artifacts in phase separation studies.

Ensuring Accuracy and Reliability in Scientific Research

This research emphasizes the importance of rigorous experimental design and careful consideration of potential confounding factors in scientific research. The study highlights the potential for commonly used reagents to have unintended consequences, urging researchers to conduct thorough investigations to ensure the accuracy and reliability of their findings.

Dr. Camel's Conclusion

Just as a mirage can deceive a thirsty traveler in the desert, seemingly innocuous reagents can distort our understanding of complex biological processes. This research serves as a valuable reminder to scrutinize our methods and ensure accuracy in our scientific explorations, particularly in the fascinating world of phase separation.
Date :
  1. Date Completed 2021-08-25
  2. Date Revised 2021-08-25
Further Info :

Pubmed ID

33814344

DOI: Digital Object Identifier

PMC7948595

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PICO Info
in preparation
Languages

English

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