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Original Abstract of the Article :
A technique involving culture in soft agar was used for the assay of forward mutation of V79 cells to 6-thioguanine (6TG) resistance. The main reason for the use of soft agar was to prevent reduction in recovery of mutants depending on the cell density plated for mutation selection, which is the chi...See full text at original site
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引用元：
https://doi.org/10.1016/0027-5107(84)90037-x
データ提供：米国国立医学図書館（NLM）
A New Method for Studying Mutations: Soft Agar Technique
Research into mutagenesis is essential for understanding the mechanisms of DNA damage and repair. This study introduces a novel method for studying forward mutations in V79 cells using a soft agar technique. The researchers aimed to eliminate the effects of metabolic co-operation, a phenomenon that can interfere with accurate measurement of mutation frequencies.
Soft Agar Technique: A More Accurate Method for Studying Mutations
The study demonstrated that V79 cells can grow well in fortified soft agar medium and that this technique allows for more accurate and quantitative measurement of mutation frequencies. The researchers found that the frequency of 6TG-resistant colonies in agar selective medium increased linearly with increasing concentrations of EMS, a known mutagen.
Implications for Mutagenesis Research
The findings highlight the advantages of the soft agar technique in studying mutagenesis. It provides a more accurate and reliable method for measuring mutation frequencies, especially at higher cell densities. This technique is a valuable tool for researchers studying DNA damage and repair.
Dr. Camel's Conclusion
This study is a valuable contribution to the field of mutagenesis research. It provides a more accurate and reliable method for studying mutations, eliminating the confounding effects of metabolic co-operation. It's like finding a hidden spring in the desert, offering a new and more accurate source of information about mutagenesis.
Date :
- Date Completed 1984-02-23
- Date Revised 2019-07-02
Further Info :
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English
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