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Intracellular activity of antibiotics against Staphylococcus aureus in a mouse peritonitis model.

Author: BlomJens, Frimodt-MøllerNiels, HesslerJonas H R, SandbergAnne, SkovRobert L

Journal ArticleAn article published in an academic or professional journal that reports original research or scholarly analysis.
Research Support, Non-U.S. Gov'tResearch funding provided by government agencies outside the United States.

Overview

Antibiotic treatment of Staphylococcus aureus infections is often challenging due to slow responses and high recurrence rates. Intracellular persistence of staphylococci has been recognized as a contributing factor. This study used a mouse peritonitis/sepsis model to explore the interplay between intracellular antibiotic activity and the overall outcome of infection. The study used a quantitative ex vivo extra- and intracellular differentiation assay to assess the intracellular presence of S. aureus, which was also confirmed by electron microscopy. Five antibiotics, dicloxacillin, cefuroxime, gentamicin, azithromycin, and rifampin (rifampicin), were tested in the new in vivo model. The intracellular effects of these antibiotics were ranked as follows, based on the mean change in the log(10) number of CFU/ml (Delta log(10) CFU/ml) between treated and untreated mice after 4 hours of treatment: dicloxacillin (3.70 Delta log(10) CFU/ml) > cefuroxime (3.56 Delta log(10) CFU/ml) > rifampin (1.86 Delta log(10) CFU/ml) > gentamicin (0.61 Delta log(10) CFU/ml) > azithromycin (0.21 Delta log(10) CFU/ml). The study also highlighted the importance of dose size, number, and frequency, time of exposure, and the timing between infection onset and treatment in testing intracellular activity in vivo. A poor correlation was found between intracellular antibiotic accumulation and the actual intracellular effect, emphasizing the need for experimental studies like those described here to measure actual intracellular activity instead of relying solely on cellular pharmacokinetic and MICs.
Paper Details　
Original Abstract of the Article :
Antibiotic treatment of Staphylococcus aureus infections is often problematic due to the slow response to therapy and the high frequency of infection recurrence. The intracellular persistence of staphylococci has been recognized and could offer a good explanation for these treatment difficulties. Kn...See full text at original site
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引用元：
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2681564/

データ提供：米国国立医学図書館（NLM）

Intracellular Activity of Antibiotics Against Staphylococcus aureus in a Mouse Peritonitis Model

This study investigates the intracellular activity of various antibiotics against Staphylococcus aureus, a common bacteria that can cause a range of infections, in a mouse model of peritonitis. It’s like studying the effectiveness of different desert plants in combating a specific type of desert pest. The study explores how antibiotics work within infected cells and their impact on the overall outcome of infection.

Understanding Antibiotic Effectiveness

The study examined the intracellular activity of five antibiotics against Staphylococcus aureus, like testing different types of desert pesticides. The researchers found that certain antibiotics, like a powerful desert insecticide, were more effective at reducing bacterial populations within cells than others. The study also highlighted the importance of understanding factors like dosage, timing, and duration of treatment, which can impact the effectiveness of antibiotics.

Combatting Drug-Resistant Infections

This research provides valuable insights into the complexities of antibiotic treatment, like navigating a desert landscape riddled with hidden dangers. The study emphasizes the need to consider both the extra- and intracellular activities of antibiotics to effectively combat drug-resistant infections. These findings could lead to the development of more effective antibiotic therapies, helping us to better combat the growing threat of antibiotic resistance.

Dr.Camel's Conclusion

This research, like a journey through a desert landscape, sheds light on the intricacies of antibiotic treatment. The study emphasizes the importance of understanding both the extra- and intracellular activities of antibiotics to effectively combat drug-resistant infections. These findings could lead to the development of more effective antibiotic therapies, helping us to better combat the growing threat of antibiotic resistance.
Date :
  1. Date Completed 2009-06-24
  2. Date Revised 2021-10-20
Further Info :

Pubmed ID

19223616

DOI: Digital Object Identifier

PMC2681564

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Languages

English

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