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Engineering, conjugation, and immunogenicity assessment of Escherichia coli O121 O antigen for its potential use as a typhoid vaccine component.

Author: CarranzaPaula, CorradinGiampietro, KowarikMichael, SteffenMichael, WackerMichael, WetterMichael

Journal ArticleAn article published in an academic or professional journal that reports original research or scholarly analysis.

Overview

The bacterial N-linked protein glycosylation system was used to produce glycoconjugates. In this report, Vi-like polysaccharide structures that can be transferred by PglB were evaluated as typhoid vaccine components. The mutant O antigen structure was transferred to acceptor proteins using the bacterial N-glycosylation system, and immunogenicity of the resulting conjugates was evaluated in mice.
Paper Details　
Original Abstract of the Article :
State-of-the-art production technologies for conjugate vaccines are complex, multi-step processes. An alternative approach to produce glycoconjugates is based on the bacterial N-linked protein glycosylation system first described in Campylobacter jejuni. The C. jejuni N-glycosylation system has been...See full text at original site
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引用元：
https://doi.org/10.1007/s10719-012-9451-9

データ提供：米国国立医学図書館（NLM）

Engineering a New Vaccine: Targeting Typhoid Fever

Typhoid fever, a serious bacterial infection, continues to be a threat to global health. This study explores a novel approach to developing a typhoid vaccine, utilizing the bacterial N-linked protein glycosylation system to engineer a conjugate vaccine. The researchers investigate the potential of using the O antigen of Escherichia coli O121, a polysaccharide that resembles the Vi antigen of Salmonella enterica serovar Typhi, as a vaccine component.

The researchers successfully engineered a mutant strain of E. coli O121 that produces an O polysaccharide reactive with antibodies against the Vi antigen. They demonstrate that this polysaccharide can be transferred to acceptor proteins using the bacterial N-linked protein glycosylation system, paving the way for the development of a novel conjugate vaccine against typhoid fever.

A Novel Approach to Vaccine Development

This study offers a promising new strategy for developing a typhoid vaccine. The researchers' innovative approach utilizes the bacterial N-linked protein glycosylation system to engineer a conjugate vaccine that may offer improved efficacy and safety.

Fighting Typhoid Fever with Innovation

The development of a novel typhoid vaccine is a crucial step toward combating this serious infectious disease. The researchers' innovative approach to vaccine engineering holds promise for improving the effectiveness of existing vaccines and potentially developing new vaccines that can provide long-lasting protection against typhoid fever.

Dr.Camel's Conclusion

This study highlights the exciting potential of bioengineering in the development of new vaccines. The researchers' innovative approach to vaccine engineering offers hope for improved protection against typhoid fever, a disease that continues to affect millions worldwide. It's a reminder that the journey toward a healthier world is an ongoing quest for innovation and advancement.

Date :
  1. Date Completed 2013-12-23
  2. Date Revised 2021-10-21
Further Info :

Pubmed ID

23053636

DOI: Digital Object Identifier

10.1007/s10719-012-9451-9

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SNS
PICO Info
in preparation
Languages

English

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