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Comparison of two hyoscyamine 6β-hydroxylases in engineering scopolamine biosynthesis in root cultures of Scopolia lurida.

Author: BaiGe, ChenMin, HuangLuqi, LanXiaozhong, LiaoZhihua, LiuKe, ZengJunlan, ZhangFangyuan

Comparative StudyResearch that compares multiple groups/interventions to determine differences/similarities in outcomes/characteristics.
Evaluation StudyResearch that systematically evaluates the effectiveness of a particular program or policy. It measures its outcomes and provides important information for future decision-making.
Journal ArticleAn article published in an academic or professional journal that reports original research or scholarly analysis.
Research Support, Non-U.S. Gov'tResearch funding provided by government agencies outside the United States.

Overview

Overexpression of hyoscyamine 6β-hydroxylase genes (HnH6H and SlH6H) in hairy root cultures of Scopolia lurida resulted in increased production of scopolamine and anisodamine. HnH6H was found to be more effective than SlH6H for engineering scopolamine production.
Paper Details　
Original Abstract of the Article :
Scopolia lurida, a medicinal plant native to the Tibetan Plateau, is among the most effective producers of pharmaceutical tropane alkaloids (TAs). The hyoscyamine 6β-hydroxylase genes of Hyoscyamus niger (HnH6H) and S. lurida (SlH6H) were cloned and respectively overexpressed in hairy root cultures ...See full text at original site
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引用元：
https://doi.org/10.1016/j.bbrc.2018.01.173

データ提供：米国国立医学図書館（NLM）

Engineering Scopolamine Biosynthesis in Root Cultures

The field of [pharmaceutical production] is constantly seeking new ways to optimize the production of valuable medicinal compounds. This study focuses on improving the biosynthesis of scopolamine, a crucial tropane alkaloid, in root cultures of Scopolia lurida. The researchers explore the potential of overexpressing hyoscyamine 6β-hydroxylase genes (H6H) from different plant sources to enhance scopolamine production.

Overexpressing HnH6H Boosts Scopolamine Production

The study reveals that overexpressing HnH6H, the H6H gene from Hyoscyamus niger, resulted in significantly higher scopolamine levels in root cultures compared to overexpressing SlH6H, the gene from S. lurida. This finding suggests that HnH6H is a more effective enzyme for engineering scopolamine production in S. lurida root cultures.

A Step Towards Sustainable Scopolamine Production

This research offers a promising approach to sustainably produce scopolamine, a valuable medicinal compound. By optimizing the biosynthesis of scopolamine in root cultures, this study provides a potential solution for the challenges associated with traditional methods of production. This advancement has the potential to contribute to the development of more efficient and sustainable pharmaceutical production processes.

Dr.Camel's Conclusion

Just as a camel carefully selects the most succulent plants in the desert, researchers are carefully choosing the right tools to optimize the production of valuable medicinal compounds. This research demonstrates the power of genetic engineering to enhance scopolamine biosynthesis, opening up new possibilities for sustainable pharmaceutical production. This is a significant step toward ensuring a reliable and efficient supply of this crucial medicinal compound.

Date :
  1. Date Completed 2018-03-23
  2. Date Revised 2022-04-08
Further Info :

Pubmed ID

29407173

DOI: Digital Object Identifier

10.1016/j.bbrc.2018.01.173

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・Effects of hyoscyamine
SNS
PICO Info
in preparation
Languages

English

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