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Response of MUTZ-3 dendritic cells to the different components of the Haemophilus influenzae type B conjugate vaccine: towards an in vitro assay for vaccine immunogenicity.

Author: HoefnagelMarcel H N, ScheperRik J, VandebrielRob J, VermeulenJolanda P

Journal ArticleAn article published in an academic or professional journal that reports original research or scholarly analysis.

Overview

MUTZ-3, a human cell line that closely resembles ex vivo human dendritic cells (DC), could be a valuable tool for in vitro immunogenicity testing. This study showed that MUTZ-3 DC can discriminate between compounds with different immunogenicity, making it a promising alternative to in vivo potency testing.
Paper Details　
Original Abstract of the Article :
Potency testing is mandatory for vaccine registration and batch release. Due to various limitations to in vivo potency testing, there is need for relevant in vitro alternatives. These alternative tests should preferably comprise cells from the target (human) species. The whole suite of immune respon...See full text at original site
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引用元：
https://doi.org/10.1016/j.vaccine.2011.05.050

データ提供：米国国立医学図書館（NLM）

Unlocking Vaccine Immunogenicity: Evaluating the Response of MUTZ-3 Dendritic Cells to Haemophilus influenzae Type B Vaccine Components

This research explores the potential of an in vitro assay using MUTZ-3 dendritic cells (DCs), a human cell line, to assess the immunogenicity of Haemophilus influenzae type b (Hib) conjugate vaccine. DCs, as important orchestrators of the immune response, are a promising candidate cell type for in vitro assays. The study compared the response of MUTZ-3 DCs to monocyte-derived DCs (moDCs), evaluating their responses to different vaccine components, including the Hib antigen (PRP), outer membrane protein (OMP), conjugated PRP-OMP, and the adjuvanted vaccine (PedVax HiB). The results showed that MUTZ-3 DCs responded consistently to different vaccine components, demonstrating their potential as a reliable in vitro model for assessing vaccine immunogenicity.

Moving Beyond Animal Testing: The Quest for In Vitro Immunogenicity Assays

This research highlights the importance of developing in vitro assays to evaluate vaccine immunogenicity, reducing the reliance on animal testing. MUTZ-3 DCs, as a stable and reproducible human cell line, offer a promising alternative for assessing vaccine effectiveness.

The Future of Vaccine Development: A Blend of In Vitro and In Vivo Techniques

While in vitro assays are valuable tools for assessing vaccine immunogenicity, they cannot completely replace in vivo testing. However, by combining in vitro and in vivo approaches, researchers can develop more robust and comprehensive methods for evaluating vaccine efficacy.

Dr. Camel's Conclusion

This study represents a significant step towards developing more efficient and ethical methods for evaluating vaccine immunogenicity. Just as a camel can find water in the desert by relying on both its physical senses and its knowledge of the environment, researchers are utilizing a combination of in vitro and in vivo approaches to understand and optimize the efficacy of vaccines.
Date :
  1. Date Completed 2012-01-18
  2. Date Revised 2011-07-18
Further Info :

Pubmed ID

21624423

DOI: Digital Object Identifier

10.1016/j.vaccine.2011.05.050

SNS
PICO Info
in preparation
Languages

English

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