Effects of dnase: A Synthesis of Findings from 25 Studies

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Original Abstract of the Article

Major research findings

DNase-1 has been shown to have protective effects in neurogenic pulmonary edema (NPE) by regulating neutrophil extracellular traps (NETs) after subarachnoid hemorrhage (SAH) in mice. 2 . DNase-1 treatment significantly reduced lung water, neutrophil infiltration, and inflammation. 2 . Furthermore, DNase-1 inhibited the formation of NETs and the proinflammatory subtype transition of macrophages. 2 . Depletion of neutrophils also confirmed the role of NETs in NPE. 2 . These findings suggest that DNase-1 has the potential to be an effective treatment for NPE after SAH. 2 .

DNase-1 can also be used for various applications in the laboratory. For example, DNase-1 can be used to digest DNA in order to study chromosomes. 18 . DNase-1 can also be used in the isolation of satellite cells from muscle tissue, which are important for muscle growth and regeneration. 4 .

Benefits and risks

Benefits summary

DNase-1 has been shown to have beneficial effects in various disease models. 7 . 3 . . For example, DNase-1 has been shown to be renoprotective in a rat model of ischemia/reperfusion-induced acute kidney injury. 7 . DNase-1 has also been shown to be protective in a rat model of intestinal ischemia-reperfusion injury. 3 . Furthermore, DNase-1 has been shown to have antitumor effects in a murine model. .

Risks summary

While DNase-1 has shown promise in various disease models, there are also potential risks associated with its use. For example, DNase-1 can also degrade proteins in addition to DNA. . Therefore, DNase-1 may have unintended effects on other cellular processes. . Furthermore, DNase-1 may affect cell differentiation and function. 4 . More research is needed to fully understand the potential risks associated with DNase-1.

Comparison of studies

Commonalities of studies

Across these studies, DNase-1 has consistently been shown to degrade DNA and have beneficial effects in various disease models. 7 . 3 . . The observed beneficial effects of DNase-1 are likely due to its ability to degrade DNA, which can contribute to inflammation and cell death.

Differences between studies

Different studies have used various methods for administering DNase-1, doses, target diseases, and evaluation criteria. 7 . 3 . . Therefore, direct comparisons between the results of these studies may not be possible. Additionally, the studies have used different animal models, which can further complicate the comparison of results. However, the results across these studies suggest that DNase-1 has potential as a therapeutic agent for various diseases.

Consistency and contradictions of results

DNase-1 has been shown to have beneficial effects in several different disease models. 7 . 3 . . However, more research is needed to understand the full range of its effects and to ensure its safety for long-term use.

Considerations for real-world application

While DNase-1 shows promise for therapeutic applications, it is important to consider that it is still under development. 7 . 3 . . Therefore, DNase-1 is currently only used in clinical trials. 7 . 3 . . More research is necessary to understand its long-term safety and efficacy. 7 . 3 . .

Limitations of current research

Research on DNase-1 is still in its early stages, and there are limitations to the available studies. 7 . 3 . . The long-term safety and efficacy of DNase-1 are not yet fully understood. 7 . 3 . .

Future research directions

More research is needed to understand the full range of effects of DNase-1 and its safety. 7 . 3 . . Specifically, further research is required to assess the long-term side effects of DNase-1 and to evaluate its effectiveness for various diseases. 7 . 3 . .

Conclusion

DNase-1 has shown promise as a therapeutic agent for a variety of diseases, including neurogenic pulmonary edema, acute kidney injury, and intestinal ischemia-reperfusion injury. 7 . 3 . . However, more research is needed to understand its safety and efficacy before it can be used widely in clinical practice. 7 . 3 . . Further studies are needed to investigate its potential risks and benefits, and to determine its optimal dosage and administration route for specific diseases. 7 . 3 . .

Literature analysis of 25 papers
Positive Content
19
Neutral Content
3
Negative Content
3
Article Type
3
0
0
0
24
1.

THE EFFECTS OF DNASE I AND LOW-MOLECULAR-WEIGHT HEPARIN IN A MURINE MODEL OF POLYMICROBIAL ABDOMINAL SEPSIS.

Author: MedeirosSarah K, SharmaNeha, DwivediDhruva, CaniErblin, ZhouJi, DwivediNaviya, SohrabipourSahar, LiawPatricia C

Sepsis treatment
Drug interaction

This study investigated the combination of DNase I and low-molecular weight heparin (LMWH) for treating sepsis in mice. While both treatments improved survival compared to saline, the combination was not more effective and potentially caused a negative drug-drug interaction.

Journal ArticleAn article published in an academic or professional journal that reports original research or scholarly analysis.
Research Support, Non-U.S. Gov'tResearch funding provided by government agencies outside the United States.

Language : English

3.

DNase-1 Treatment Exerts Protective Effects in a Rat Model of Intestinal Ischemia-Reperfusion Injury.

Author: WangShikai, XieTian, SunShilong, WangKai, LiuBaochen, WuXingjiang, DingWeiwei

Intestinal I/R injury treatment

Neutrophil extracellular traps (NETs) are structures released by neutrophils that can contribute to inflammation and cell death. This study found that NETs are involved in intestinal I/R injury and that DNase-1, an enzyme that breaks down DNA, can reduce the severity of the injury.

Journal ArticleAn article published in an academic or professional journal that reports original research or scholarly analysis.
Research Support, Non-U.S. Gov'tResearch funding provided by government agencies outside the United States.

Language : English

6.

Antitumor Effects of Systemic DNAse I and Proteases in an In Vivo Model.

Author: Trejo-BecerrilCatalina, Pérez-CardenasEnrique, Gutiérrez-DíazBlanca, De La Cruz-SigüenzaDesiree, Taja-ChayebLucía, González-BallesterosMauricio, García-LópezPatricia, ChanonaJosé, Dueñas-GonzálezAlfonso

Antitumor effects

This study explores the antitumor effects of DNAse I and a protease mix on circulating cell-free DNA (cfDNA) in cancer patients. The enzymes effectively degraded cfDNA in vitro and in vivo, showing potential for inhibiting tumor growth.

Journal ArticleAn article published in an academic or professional journal that reports original research or scholarly analysis.
Research Support, Non-U.S. Gov'tResearch funding provided by government agencies outside the United States.

Language : English

7.

Renoprotective Effects of DNAse-I Treatment in a Rat Model of Ischemia/Reperfusion-Induced Acute Kidney Injury.

Author: PeerVictoria, Abu HamadRamzia, BermanSylvia, EfratiShai

Renoprotective effects
Acute kidney injury

Accumulation of cell-free DNA debris is a marker of organ damage. This study investigated whether exogenous DNAse-I administration could exert renoprotective effects in acute kidney injury (AKI).

Journal ArticleAn article published in an academic or professional journal that reports original research or scholarly analysis.
Research Support, Non-U.S. Gov'tResearch funding provided by government agencies outside the United States.

Language : English

8.

Effects of Nephrolithiasis on Serum DNase (Deoxyribonuclease I and II) Activity and E3 SUMO-Protein Ligase NSE2 (NSMCE2) in Malaysian Individuals.

Author: YusofFaridah, MehdeAtheer Awad, MehdiWesen Adel, RausRaha Ahmed, GhazaliHamid, RahmanAzlina Abd

Kidney damage biomarker

This study explored the potential of DNase I/II activity and E3 SUMO-protein ligase NSE2 as biomarkers for kidney damage in nephrolithiasis patients.

Journal ArticleAn article published in an academic or professional journal that reports original research or scholarly analysis.
Research Support, Non-U.S. Gov'tResearch funding provided by government agencies outside the United States.

Language : English

9.

Ca2+, Mg2+-dependent DNase involvement in apoptotic effects in spermatozoa of sea urchin Strongylocentrotus intermedius induced by two-headed sphingolipid rhizochalin.

Author: SibirtsevJuriy T, ShastinaValeria V, MenzorovaNatalia I, MakarievaTatyana N, RasskazovValeriy A

The study found that rhizochalin (Rhz), a two-headed sphingolipid, induced apoptosis in sea urchin spermatozoa. This process involved activation of caspase-3, which likely activated Ca(2+), Mg(2+)-dependent DNase (Ca, Mg-DNase), leading to internucleosomal DNA cleavage. This suggests that Ca, Mg-DNase plays a key role in the apoptotic process in sea urchin spermatozoa.

Comparative StudyResearch that compares multiple groups/interventions to determine differences/similarities in outcomes/characteristics.
Journal ArticleAn article published in an academic or professional journal that reports original research or scholarly analysis.
Research Support, Non-U.S. Gov'tResearch funding provided by government agencies outside the United States.

Language : English

10.

F-actin structure destabilization and DNase I binding loop: fluctuations mutational cross-linking and electron microscopy analysis of loop states and effects on F-actin.

Author: Oztug DurerZeynep A, DiraviyamKarthikeyan, SeptDavid, KudryashovDmitri S, ReislerEmil

Improved actin stability

The conformational dynamics of filamentous actin (F-actin) are essential for the regulation and functions of cellular actin networks.

Journal ArticleAn article published in an academic or professional journal that reports original research or scholarly analysis.
Research Support, N.I.H., ExtramuralResearch funding provided by the NIH to external research institutions and researchers.
Research Support, U.S. Gov't, Non-P.H.S.Research funding provided by US government agencies other than the Public Health Service.

Language : English

11.

Effects of recombinant human DNase and hypertonic saline on airway inflammation in children with cystic fibrosis.

Author: SuriRanjan, MarshallLindsay J, WallisColin, MetcalfeChristopher, BushAndrew, ShuteJanis K

Mucolytic effect

rhDNase and hypertonic saline are effective mucolytic therapies in cystic fibrosis, but their effects on airway inflammation are not fully understood. This study compared the effects of rhDNase and hypertonic saline on cationic proinflammatory mediators in CF sputum. The study found that neither rhDNase nor hypertonic saline promoted airway inflammation in CF patients.

Clinical TrialA clinical trial is a planned study conducted on humans to evaluate the efficacy and safety of pharmaceuticals, medical devices, and treatment methods. It aims to explore the possibilities of new treatments and improve the health of patients.
Comparative StudyResearch that compares multiple groups/interventions to determine differences/similarities in outcomes/characteristics.
Journal ArticleAn article published in an academic or professional journal that reports original research or scholarly analysis.
Randomized Controlled TrialA clinical trial that randomly assigns participants to treatment and control groups to evaluate the effects of an intervention.
Research Support, Non-U.S. Gov'tResearch funding provided by government agencies outside the United States.

Language : English

12.

Evaluation of the effects of DNase treatment on signal specificity in RT-PCR and in situ RT-PCR.

Author: IvarssonK, WeijdegårdB

The effects of DNase treatment on the specificity of reverse transcription (RT)-PCR were investigated. Direct in situ RT-PCR is an unreliable method because the necessary DNase treatment induces nonspecific amplification.

Research Support, Non-U.S. Gov'tResearch funding provided by government agencies outside the United States.
Technical ReportA document that reports the results of technical or scientific research in detail. It details the methodology, results, and conclusions.

Language : English

13.

Combined effects of interferon-alpha and acyclovir on herpes simplex virus type 1 DNA polymerase and alkaline DNase.

Author: TaylorJ L, TomP, O'BrienW J

HSV-1 treatment

Combination treatment with IFN-alpha and ACV synergistically inhibited herpes simplex virus type 1 replication. This synergy may be mediated by IFN-alpha's effect on the production of viral early enzymes, particularly DNA polymerase.

Journal ArticleAn article published in an academic or professional journal that reports original research or scholarly analysis.
Research Support, Non-U.S. Gov'tResearch funding provided by government agencies outside the United States.
Research Support, U.S. Gov't, P.H.S.Research funding provided by the Public Health Service (PHS).

Language : English

14.

Analysis of long-range structural effects induced by DNase-I interaction with actin monomeric form or complexed to CapZ.

Author: UsmanovaA, AstierC, LebartM C, KwiatekO, PapaI, BoyerM, RoustanC, BenyaminY

DNase-I binding to actin induced structural changes that decreased CapZ binding and abolished DNase-I inhibition activity.

Journal ArticleAn article published in an academic or professional journal that reports original research or scholarly analysis.
Research Support, Non-U.S. Gov'tResearch funding provided by government agencies outside the United States.

Language : English

15.

Effects of non-conservative changes to tyrosine 76, a key DNA binding residue of DNase I, on phosphodiester bond cleavage and DNA hydrolysis selectivity.

Author: WarrenM A, EvansS J, ConnollyB A

DNA binding
DNA cutting

Non-conservative mutations to tyrosine 76, a key DNA binding residue in DNase I, resulted in tighter DNA binding but slower DNA cleavage.

Journal ArticleAn article published in an academic or professional journal that reports original research or scholarly analysis.
Research Support, Non-U.S. Gov'tResearch funding provided by government agencies outside the United States.

Language : English

17.

In vivo effects of recombinant human DNase I on sputum in patients with cystic fibrosis.

Author: ShahP L, ScottS F, KnightR A, MarriottC, RanasinhaC, HodsonM E

Improved pulmonary function
Reduced sputum viscoelasticity

This study evaluated the in vivo effects of recombinant human DNase I (rhDNase) on sputum rheology and pulmonary function in patients with cystic fibrosis. RhDNase was found to significantly reduce sputum viscoelasticity and improve pulmonary function, suggesting that rhDNase may be a valuable treatment for improving airway clearance and lung function in CF patients.

Clinical TrialA clinical trial is a planned study conducted on humans to evaluate the efficacy and safety of pharmaceuticals, medical devices, and treatment methods. It aims to explore the possibilities of new treatments and improve the health of patients.
Journal ArticleAn article published in an academic or professional journal that reports original research or scholarly analysis.
Randomized Controlled TrialA clinical trial that randomly assigns participants to treatment and control groups to evaluate the effects of an intervention.

Language : English

18.

Treatments with saline solutions and DNase I have different effects on DNA content and distribution in human and in mouse chromosomes.

Author: BottoneM G, MalgaraR, PellicciariC, Fuhrman ContiA M

Chromosome analysis
G-banding procedure

Treatment of chromosomes with saline solutions induced a G-banding-like pattern after Giemsa staining without significant displacement of genomic DNA. This method allows for the classification of single chromosomes without affecting further analysis in situ.

Comparative StudyResearch that compares multiple groups/interventions to determine differences/similarities in outcomes/characteristics.
Journal ArticleAn article published in an academic or professional journal that reports original research or scholarly analysis.
Research Support, Non-U.S. Gov'tResearch funding provided by government agencies outside the United States.

Language : English

19.

Effects of recombinant human DNase therapy on healthcare use and costs in patients with cystic fibrosis.

Author: OsterG, HuseD M, LaceyM J, ReganM M, FuchsH J

Cystic fibrosis treatment

rhDNase therapy reduced the costs of treating RTIs in patients with cystic fibrosis; assuming once-daily dosing, these savings would offset about one-third of the cost of such therapy.

Clinical TrialA clinical trial is a planned study conducted on humans to evaluate the efficacy and safety of pharmaceuticals, medical devices, and treatment methods. It aims to explore the possibilities of new treatments and improve the health of patients.
Clinical Trial, Phase IIIA Phase III trial is a trial that further rigorously verifies the efficacy and safety of a new drug or treatment, whose efficacy and safety have been confirmed in a Phase II trial, in a large number of patients, and evaluates whether it is superior to existing treatments.
Journal ArticleAn article published in an academic or professional journal that reports original research or scholarly analysis.
Multicenter StudyResearch conducted in collaboration with multiple research institutions. It aims to collect a wide range of data and obtain more generalizable results.
Randomized Controlled TrialA clinical trial that randomly assigns participants to treatment and control groups to evaluate the effects of an intervention.
Research Support, Non-U.S. Gov'tResearch funding provided by government agencies outside the United States.

Language : English

20.

Chromosomal position effects in chicken lysozyme gene transgenic mice are correlated with suppression of DNase I hypersensitive site formation.

Author: HuberM C, BoschF X, SippelA E, BoniferC

Gene expression

The complete chicken lysozyme gene locus was found to be expressed copy number dependently in transgenic mice, demonstrating the importance of all cis regulatory elements for position-independent gene expression.

Journal ArticleAn article published in an academic or professional journal that reports original research or scholarly analysis.
Research Support, Non-U.S. Gov'tResearch funding provided by government agencies outside the United States.

Language : English

21.

Effects of isoflurane on the DNase I activity in an isolated enzyme preparation and on the DNase I-G actin complex.

Author: ReitzM, von PfeilA, HundtF, LanzE

DNA damage

Isoflurane inhibited DNase I activity at clinically relevant concentrations.

Journal ArticleAn article published in an academic or professional journal that reports original research or scholarly analysis.
Research Support, Non-U.S. Gov'tResearch funding provided by government agencies outside the United States.

Language : English

22.

The effects of enflurane on the DNase I activity in an isolated enzyme preparation and in the DNase I-G actin complex.

Author: ReitzM, Leicher-DüberA, LanzE

Anaesthetic side effects

Enflurane altered the conformation of DNase I without inhibiting its function. This suggests that enflurane may have side effects related to its interactions with proteins.

Journal ArticleAn article published in an academic or professional journal that reports original research or scholarly analysis.
Research Support, Non-U.S. Gov'tResearch funding provided by government agencies outside the United States.

Language : English

24.

Effects of DNase production, plasmid size, and restriction barriers on transformation of Vibrio cholerae by electroporation and osmotic shock.

Author: MarcusH, KetleyJ M, KaperJ B, HolmesR K

Transformation efficiency improvement

Electroporation is an effective method for transforming V. cholerae, overcoming the barrier of extracellular DNase.

Journal ArticleAn article published in an academic or professional journal that reports original research or scholarly analysis.
Research Support, U.S. Gov't, P.H.S.Research funding provided by the Public Health Service (PHS).

Language : English

25.

Structural effects in axoplasm of DNase I, an actin depolymerizer that blocks fast axonal transport.

Author: NemhauserI, GoldbergD J

Axonal transport inhibition

Microtubules and neurofilaments are intermingled in axons. DNase I, an actin depolymerizer, causes localized losses of microtubules, inhibiting fast axonal transport.

Journal ArticleAn article published in an academic or professional journal that reports original research or scholarly analysis.
Research Support, U.S. Gov't, P.H.S.Research funding provided by the Public Health Service (PHS).

Language : English

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